Coacervation – a process where macromolecules phase-separate into dense liquid-like droplets – has significant applications in a variety of fields, including drug delivery and biomaterials, and is also widely studied as a key phenomenon in the origins of life. This PhD project focuses on the formation and dynamics of peptide-based coacervates as model protocells, investigating how their properties depend on peptide length, sequence, and environmental conditions.
We will first explore the minimal length and sequence requirements for peptide coacervation. Next, the project will assess how these coacervates influence the synthesis of polypeptides. In parallel, we will investigate whether polypeptide synthesis can directly trigger coacervation in situ, linking polymerization and phase separation. Finally, coacervate degradation via proteolysis will be studied, allowing us to establish dynamic cycles of coacervate growth and decay, selectively favoring the formation of specific polypeptides. By bridging physical chemistry, soft matter, polymer science, and prebiotic chemistry, this research will provide new insights into how dynamic compartmentalization could have influenced the early evolution of biomolecules.
